Cell cycle analysis by combining the 5-bromodeoxyuridine/33258 hoechst technique with DNA-specific ethidium bromide staining

BrdU-Hoechst-ethidium bromide (EB) quenching technique for studying kinetics of hematopoiesis.

Possible errors in the interpretation of ethidium bromide and PicoGreen DNA staining results from ethidium monoazide-treated DNA.

Nocker and Camper reported that DNA from dead bacterial cells (Escherichia coli O157:H7 and Salmonella enterica serovar Typhimurium) treated with ethidium monoazide (EMA) is lost during the DNA extraction procedure (2). This was concluded from the missing or less intense DNA band on an agarose gel stained with ethidium bromide in comparison to the intensities of bands in untreated samples. Acco...

Interaction of Hoechst 33258 and ethidium with histone1-DNA condensates.

We report structural and dynamical aspects of DNAs from various sources including synthetic oligonucleotides in bulk buffer and as a complex with histone1 (H1). High-resolution transmission electron microscopic (HRTEM) studies reveal the structural change of the DNAs upon complexation with H1 leading to formation of compact-globular and hollow-toroidal particles. In order to explore the functio...

A Comparative Docking Analysis of Non-Carcinogenic DNA Staining Dyes to Propose the Best Alternative to Ethidium Bromide

Fluorescent dyes that stain a cell’s DNA are used in live cell imaging as they allow for tracking of cell division, for the visualization and sizing of dsDNA restriction fragments, and for the examination of properties of the isolated DNA molecules. Conventionally, Ethidium bromide (EtBr) is the cationic dye used to visualize DNA after separating the fragments on Agarose Gel Electrophoresis. It...

Specific Inhibition by Ethidium Bromide of Mitochondrial Dna Synthesis in Physarum Polycephalum

Eucaryotic cells contain two complete genetic systems, one based on nuclear DNA and the other on cytoplasmic DNA . The study of these systems has been greatly facilitated by the existence of specific inhibitors . One such inhibitor is the trypanocidal drug, ethidium bromide (Short, Peak, and Watkins, 1953) . Application of the drug to trypanosomes in vitro causes alterations in the structure of...